Journal: International Journal of Molecular Sciences
Article Title: In Vitro Identification of New Transcriptomic and miRNomic Profiles Associated with Pulmonary Fibrosis Induced by High Doses Everolimus: Looking for New Pathogenetic Markers and Therapeutic Targets
doi: 10.3390/ijms19041250
Figure Lengend Snippet: Protein levels of collagen1 and CTGF in NIH/3T3 cells. ( A ) Representative western blotting experiments for collagen1 and CTGF. Histograms represent the mean ± SD of ( B ) collagen1 and ( C ) CTGF protein levels. GAPDH was included as loading control. ** p < 0.001, * p < 0.05 vs. CTR.
Article Snippet: Membranes were exposed to primary antibodies directed against GAPDH (Santa Cruz sc-25778), CTGF (NovusBio, Littleton, CO, USA) and collagen1 (ORIGENE TA309096) (overnight at 4 °C) and incubated with a secondary peroxidase-conjugated antibody for 1 h at room temperature.
Techniques: Western Blot